*This list also available in Microsoft Word and PDF format

BIBLIOGRAPHY

 

Publications, Summary

 

Full papers Published & In Press:                   68

                         Submitted:                               1

                          In Preparation:                        2

 

Total citations (source: Web of Knowledge 1976-present) approx. 1390

 

 

MOST SIGNIFICANT CONTRIBUTIONS

Student names *; PDF names **; James Chair Profs underlined; {funding sources}

 

1. Marshall, W.S., **Katoh, F., *Main, H.P., *Sers, N., and Cozzi R.R.F.  Focal adhesion kinase and β1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus. Comp. Biochem. Physiol. 150A:288-300 (2008) PDF

  This paper combines immunogold TEM, confocal immunocytochemistry, coimmunoprecipitation and Western blots to prove the metabolon type regulation of NKCC, the ubiquitous cotransport protein and its responsiveness to small osmotic perturbations.  This novel association of FAK with integrin and NKCC as the force sensor in the cascade builds on our 2005 paper and consolidates the role of FAK pY407 phosphorylation as central to the regulation.  Reviewers remarks: " provides evidence of an exciting and novel role for the focal adhesion kinase signaling system in osmoregulation", " the immuno-EM, in particular, is beautiful and very convincing " and " The results provided important contribution to understand the signal pathways related to NaCl secretion "{NSERC, StFX UCR, HRSDC}

2. Marshall, W.S., C.G. Ossum and E.K. Hoffmann.  Hypotonic shock mediation by p38 MAPK, JNK, PKC, FAK, OSR1 and SPAK in osmosensing chloride secreting cells of killifish opercular epithelium.  J. exp. Biol. 208:1063-1077 (2005). PDF

  Hypotonic shock from the basal (blood) side only produces abrupt inhibition of Cl- secretion and  hypertonic shock does the reverse.  In this collaborative project, we used Western analyses, pharmacology and immunocytochemistry with colocalization to reveal a kinase cascade that terminates on the cotransporter Na+,K+,2Cl- cotransporter NKCC, including FAK (focal adhesion kinase) and stress associated protein kinase (SPAK).  This is the first time FAK has been associated with ion transport and cell volume regulation.  The osmosensor that may be a key to understanding regulatory volume decrease we think involves FAK as a regulator protein that permits a kinase cascade ending with SPAK that directly phosphorylates NKCC.  Reviewer #1:"This is an important work...." {NSERC, CFI, James Chair Endowment}

3. Marshall, W.S., E.M. Lynch* and R.R.F. Cozzi. Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.  J. exp. Biol. 205: 1265-1273 (2002). PDF

  Immunocytochemistry and confocal microscopy was used here to trace where in the ion transporting cells were the main Cl transporting proteins CFTR and NKCC.  The paper clearly showed changes in transporter distribution that coincide with the appearance of Cl transepithelial transport at 24h-48h after transfer to sea water.  This slow redistribution is not simple fusion of membrane vesicles but rather appears to involve de novo expression of transporters and insertion in the plasma membrane as well as removal of the old transporter, a real “retooling” of existing chloride cells. {NSERC, CFI}

4. Marshall, W.S., S.E. Bryson and T. Luby*.  Control of epithelial Cl- secretion by basolateral osmolality in the euryhaline teleost, Fundulus heteroclitus.  J. exp. Biol. 203:1897-1905. (2000). PDF

  Hypotonic shock inhibits Cl- secretion involving protein tyrosine kinase but apparently not calcium.  This paper led to Daborn's work with pavement cell-chloride cell interactions and collaboration with E.K. Hoffmann.  It was the first to address the paradox that here transepithelial Cl- transport is inhibited by hypotonic shock, whereas regulatory volume decrease is normally associated instead with increases Cl- channel activity. {NSERC + UCR}

 5. Marshall, W.S., T.R. Emberley* S.E. Bryson and S.D. McCormick. Time course of salinity adaptation in a strongly euryhaline estuarine teleost Fundulus heteroclitus: A multivariable approach. J. exp. Biol. 202:1535-1544 (1999). PDF {NSERC + James Chair Endowment}

This paper used eight sampling times (one hour to 4 weeks) and twelve measured variables, molecular through physiological, to build for the first time a clear picture of the sequence and relationships between seawater adaptational events in an estuarine teleost.  Important time lines of events (e.g. cortisol peaks at 1 hour and kfCFTR expression increases at 8h+) were established which led to many new interesting hypotheses being generated.  This paper showed that measurement of many variables in a time course to be a much more powerful approach than the study of fully acclimated animals.

6. Marshall, W.S., R.M. Duquesnay*, J.M. Gillis*, S.E. Bryson and C.M. Liedtke. Neural modulation of salt secretion in teleost opercular epithelium by a2-adrenergic receptors and inositol 1,4,5-trisphosphate. J. exp. Biol 201:1959-1965 (1998). {NSERC + James Chair Research Grant} Here we used a unique nerve-epithelium in vitro preparation to measure neural effects on Cl- secretion and revealed that salt secretion is under neural control by the autonomic nervous system. It strengthened the conclusion of Ca2+ mediation by detecting a peak in IP3.  It supports the novel idea that changes in salinity experienced by estuarine fish are NOT necessarily stressful, rather that they can be handled with same facility as are many “higher” autonomic functions, such as sweating in primates.

7. Marshall, W.S., S.E. Bryson, A. Midelfart and W.F. Hamilton. Low conductance anion channel activated by cAMP in teleost Cl‑ secreting cells. Am. J. Physiol. 268:R963‑R969 (1995). This paper reveals presence of the anion channel CFTR in fish. It has finally allowed molecular descriptions of chloride cell operation and is unique in its cellular regulation, making the work of importance to Cystic Fibrosis research.  It paved the way for kfCFTR sequencing (see Singer et al 1998, below).

8. Marshall, W.S., S.E. Bryson and D. Garg*. a2‑adrenergic inhibition of chloride transport by opercular epithelium is mediated by intracellular Ca2+. Proc. Nat. Acad. Sci. U.S.A. 90:5504‑5508 (1993).

This paper shows a unique Ca2+‑mediated inhibition by a2‑receptors. Most receptors of this type in the vertebrates are mediated differently, via decrease in cAMP.  Hence our results run counter to the  accepted dogma at the time, but appear to be robust, as other examples have now been found.

 

LIST OF RESEARCH CONTRIBUTIONS (most recent first)

FULL PAPERS IN REFEREED JOURNALS (includes the eight above)

1.        Marshall, W.S., K.D. *Watters, R.R.F. Cozzi, and F. **Katoh. CFTR Cl- channel functional regulation by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensitive ion transporting mitochondria rich cells of euryhaline killifish.  Amer. J. Physiol.  (Submitted Dec 11. 2008)

2.        **Katoh, F., R.R.F. Cozzi, W. S. Marshall and G. G. Goss.  Distinct Na+/K+/2Cl- cotransporter localization in kidneys and  gills of two euryhaline species, rainbow trout and killifish. Cell Tiss. Res. (submitted Aug 17, 2007; accepted July 1 2008, in press.){NSERC}

3.        Marshall, W.S., **Katoh, F., *Main, H.P., *Sers, N., and Cozzi R.R.F.  Focal adhesion kinase and β1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus. Comp. Biochem. Physiol. 150A:288-300 (2008) PDF

4.        Burnett, K.G., Bain, L.J., Baldwin, W.S., Callard,G.V., Cohen, S., Di Giulio, R.T., Evans, D.H., Gómez-Chiarri, M., Hahn, M.E., Hoover, C.A., Karchner, S.I., **Katoh, F., MacLatchy, D.L., Marshall, W.S., Meyer, J.N., Nacci, D.E., Oleksiak, M.F., Rees, B.B., D. Singer, T.D., Stegeman, J.J., Towle, D.W., Van Veld, P.A., Vogelbein, W.K., Whitehead, A., Winn, R.N., Crawford, D.L. Fundulus as the premier teleost model in environmental biology: Opportunities for new insights using genomics.  Comp. Biochem. Physiol. Part D Genomics and Proteomics 2:257-286 (2007). PDF

5.        Marshall, W.S., R.F.F. Cozzi, R.M. Pelis and S.D. McCormick Cortisol receptor blockade and seawater adaptation in the euryhaline teleost Fundulus heteroclitus.  J. Exp. Zool. 303A:132-142 (2005). {NSERC James Chair and NSF} PDF

6.        Marshall, W.S., C.G. Ossum and E.K. Hoffmann.  Hypotonic shock mediation by p38 MAPK, JNK, PKC, FAK, OSR1 and SPAK in osmosensing chloride secreting cells of killifish opercular epithelium.  J. exp. Biol. 208:1063-1077 (2005) {NSERC James Chair and CFI}. PDF

7.        Marshall, W.S.  Rapid regulation of NaCl secretion by estuarine teleost fish: Coping strategies for short duration fresh water exposures.  Biochim. Biophys. Acta Biomembranes "Anion Transport" 1618:95-105 (2003) PDF

8.        Marshall, W.S., J.A. Howard*, R.R.F. Cozzi, and E.M. Lynch* NaCl and fluid secretion by the intestine of the teleost Fundulus heteroclitus: Involvement of CFTR.  J. exp. Biol. 205:745-758 (2002) PDF

9.        Marshall, W.S., E.M. Lynch* and R.F. Cozzi. Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water.  J. exp. Biol. 205: 1265-1273 (2002). PDF

10.    Daborn*, K., R.R.F. Cozzi and W.S. Marshall.  Dynamics of pavement cell-chloride cell interactions during abrupt salinity change in Fundulus heteroclitus.  J. exp. Biol. 204:1889-1899 (2001) PDF

11.    Marshall, W.S., S.E. Bryson and T. *Luby.  Control of epithelial Cl- secretion by basolateral osmolality in the euryhaline teleost, Fundulus heteroclitus.  J. exp. Biol. 203:1897-1905. (2000).

12.    Marshall, W.S., T.R. Emberley*, S.E. Bryson and S.D. McCormick.  Time course of salinity adaptation in a strongly euryhaline estuarien teleost Fundulus heteroclitus: A multivariable approach.  J. exp. Biol. 202:1535-1544 (1999). PDF

13.    Singer, T.D., S.J. Tucker, W.S. Marshall and C.F. Higgins.  A divergent CFTR homologue: Highly regulated salt transport in the euryhaline teleost Fundulus heteroclitus.  Amer. J. Physiol. 274:C715-C723 (1998). PDF

14.    Burgess, D.W., W.S. Marshall and C.M. Wood.  Ionic transport by the opercular epithelia of freshwater acclimated tilapia (Oreochromis niloticus) and killifish (Fundulus heteroclitus). Comp. Biochem. Physiol. 121A:155-164 (1998).

15.    Marshall, W.S., *DuQuesnay, R.M., *Gillis, J.M. Bryson, S.E. and C.M. Liedtke.  Neural modulation of salt secretion in teleost opercular epithelium by a2-adrenergic receptors and inositol 1,4,5-trisphosphate.  J. exp. Biol. 201:1959-1965 (1998)

16.    Patrick, M.L., Part, P., Marshall, W.S. and C.M. Wood.  Characterization of ion and acid-base transport in fresh water adapted mummichog (Fundulus heteroclitus).  J. Exp. Zool. 279:208-219 (1997).

17.    Marshall, W.S., S.E. Bryson, P. *Darling, C. *Whitten, M. Wilkie, C.M. Wood and J. Buckland-Nicks.  NaCl transport and ultrastructure of opercular epithelium from a freshwater adapted euryhaline teleost, Fundulus heteroclitus J. Exp. Zool. 277:23-37 (1997).

18.    Verbost, P.M., S.E. Bryson, S.E. Wendelaar Bonga and W.S. Marshall.   Na+-dependent Ca2+ uptake in isolated opercular epithelium of Fundulus heteroclitus.  J. Comp. Physiol. B. 167:205-212 (1997).

19.    Patrick, M.L., C.M. Wood and W.S. Marshall.  Calcium regulation in the freshwater adapted mummichog (Fundulus heteroclitus). J. Fish Biol. 51:135-145, (1997).

20.    Patrick, M.L., Part. P., Marshall, W.S. and C.M. Wood.  Ionoregulation and acid-base balance in the freshwater adapted mummichog (Fundulus heteroclitus). J. Exp. Zool. 279:208-219 (1997).

21.    Marshall, W.S., S.E. Bryson, A. Midelfart and W.F. Hamilton.  Low conductance anion channel activated by cAMP in teleost Cl- secreting cells.  Am. J. Physiol. 268:R963-R969 (1995).

22.    Marshall, W.S., S.E. Bryson, J.S. *Burghardt and P.M. Verbost.  Ca2+ transport by opercular epithelium of the freshwater adapted euryhaline teleost Fundulus heteroclitus. J. Comp. Physiol. B.  165:268-277 (1995).

23.    Wood, C.M. and W.S. Marshall.  Ion balance, acid-base regulation, and chloride cell function in the common killifish Fundulus heteroclitus - a freely euryhaline estuarine teleost.  Estuaries 17:34-52 (1994).

24.    Marshall, W.S., S.E. Bryson and D. *Garg.  α2-adrenergic inhibition of chloride transport by opercular epithelium is mediated by intracellular Ca2+.  Proc. Nat. Acad. Sci. U.S.A. 90:5504-5508 (1993).

25.    Marshall, W.S., S.E. Bryson and D.R. Idler  Gonadotropin action on sperm duct epithelium: Ion transport stimulation mediated by cAMP and Ca2+. Gen. Comp. Endocrinol. 90:232-242 (1993).

26.    Marshall, W.S., S.E. Bryson and C.M. Wood.  Calcium transport by isolated skin of rainbow trout.  J. exp. Biol. 166:297-316 (1992).

27.    Marshall, W.S. and J.W. Hanrahan.  Anion channels in the apical membrane of mammalian corneal epithelium primary cultures.  Invest. Ophthalmol. Vis. Sci. 32:1562-1568 (1991).

28.    Marshall, W.S. and S.E. Bryson.  Intracellular pH regulation in trout urinary bladder epithelium: Na+-H+(NH4+) exchange.  Am. J. Physiol. 261:R652-R658 (1991).

29.    Marshall, W.S., S.E. Bryson and M.M. *Sapp.  Volume regulation in glutathione-treated brook trout (Salvelinus fontinalis) erythrocytes.  Fish Physiol. Biochem. 8:19-28 (1990).

30.    Marshall, W.S., S.E. Bryson, and D.R. Idler.  Gonadotropin stimulation of K+ secretion and Na+ absorption by brook trout (Salvelinus fontinalis) sperm duct epithelium.  Gen. Comp. Endocrinol. 75: 118-128 (1989).

31.    Marshall, W.S.  Passive solute and fluid transport in brook trout urinary bladder epithelium. Can. J. Zool. 66:912-918 (1988).

32.    Marshall, W.S. and S.E. Bryson.  Evidence for Cl--dependent K+ secretion by the blood-testis barrier of brook trout.  Can. J. Zool. 66:1603-1609 (1988).

33.    Marshall, W.S.  Independent Na+ and Cl‑ active transport by urinary bladder epithelium of brook trout.  Am. J. Physiol.  250:R227‑R234 (1986).

34.    Lessman, C.A., W.S. Marshall and H.R. Habibi.  Nuclear movement and dissolution during Rana oocyte meiosis:  Effect of demecolcine.  Gamete Res.  14:11‑23 (1986)

35.    Marshall, W.S.  Sperm duct epithelium of brook trout:  Na+ transport and seminal plasma composition.  Can. J. Zool. 64:1827-1830 (1986).

36.    Marshall, W.S.  Paracellular ion transport in trout opercular epithelium models osmoregulatory effects of acid precipitation.  Can. J. Zool.  63:1816‑1822 (1985).

37.    Marshall, W.S., H.R. Habibi and C.A. Lessman.  Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout (Salvelinus fontinalis). Can. J. Zool.  63:1904‑1908 (1985).

38.    Lessman, C.A. and W.S. Marshall.  Electrophysiology of insulin‑ and progesterone‑induced meiosis in Rana pipiens oocytes.  J. Exp. Zool.  231:257‑266, (1984).

39.    Marshall, W.S. and S.D. Klyce.  Cellular mode of serotonin action in the rabbit corneal epithelium.  Biochim. Biophys. Acta., 778:139‑143 (1984).

40.    Marshall, W.S. and S.D. Klyce.  Membrane and shunt resistances in the rabbit corneal epithelium.  J. Memb. Biol.  73:275‑283 (1983).

41.    Klyce, S.D. and W.S. Marshall.  Effects of Ag+ on ion transport by the rabbit corneal epithelium.  J. Memb. Biol. 66:133‑145 (1982).

42.    Klyce, S.D., K.A. Palkama, A.H. Neufeld, M. Harkonen, W.S. Marshall, S.  Hutaniitty, and K.P. Mann.  Neural serotonin stimulates chloride transport in the rabbit corneal epithelium.  Invest. Ophthalmol. Vis. Sci. 23:181‑192 (1982).

43.    Marshall, W.S. and S.D. Klyce.  Numerical procedures for ion‑selective electrodes.  J. Electrophysiol. Tech. 9:113‑121 (1982).

44.    Marshall, W.S. and Bern, H.A.  Active chloride transport by the skin of a marine teleost is stimulated by urotensin I and inhibited by urotensin II.  Gen. Comp. Endocrinol., 43:484‑491 (1981).

45.    Bern, H.A., C.A. Bisbee, N.L. Collie, J.K. Foskett, B. Hughes, C.A. Loretz and W.S. Marshall.  Failure of ovine prolactin to elicit rapid effects on osmoregulatory surfaces.  Gen. Comp. Endocrinol. 44:128‑130 (1981).

46.    Marshall, W.S. and S.D. Klyce.  Cell finder speeds impalements with microelectrodes.  Pflugers Archiv 391:258‑259 (1981).

47.    Marshall, W.S.  Sodium dependency of active chloride transport across isolated fish skin (Gillichthys mirabilis).  J. Physiol. Lond. 319:165‑178 (1981).

48.    Marshall, W.S.  Active transport of Rb+ across the skin of the teleost Gillichthys mirabilis.  Am. J. Physiol. 241:F482‑F486 (1981).

49.    Marshall, W.S. and Nishioka, R.S.  Relation of mitochondria‑rich chloride cells to anion transport by marine teleost skin.  J. Exp. Zool., 214:147‑156 (1980).

50.    Marshall, W.S. and Bern, H.A.  Teleostean urophysis:  urotensin II and ion transport across the isolated skin of a marine teleost.  Science 204:519‑521 (1979).

51.    Marshall, W.S.  Effects of salinity acclimation, ovine prolactin, and cortisol on the mucus cells of Leptocottus armatus (teleostei:cottidae).  Gen. Comp. Endocrinol. 37:358‑368 (1979).

52.    Marshall, W.S.:  On the involvement of mucus secretion in teleost osmoregulation.  Can. J. Zool. 56:1088‑1091 (1978).

53.    Marshall, W.S.:  Transepithelial potential and short‑circuit current across the isolated skin of Gillichthys mirabilis (teleostei: gobiidae), acclimated to 5% and 100% sea water.  J. Comp. Physiol. 114:157‑165 (1977).

54.    Marshall, W.S.:  Effects of hypophysectomy and ovine prolactin on the epithelial mucus‑secreting cells of the pacific staghorn sculpin, Leptocottus armatus (teleostei: cottidae).  Can. J. Zool. 54:1604‑1609 (1976).

 

BOOK CHAPTERS AND REVIEWS CONTAINING NEW DATA

 

55.    Marshall, W.S. Osmoregulation and ion balance in health and disease In: Fish Diseases,  J. Leatherland, Ed. To appear  2009

56.    Marshall, W.S. Kerala chapter 2009

57.    Hoffmann, E.K., T. Schettino and W.S. Marshall.  The role of volume-sensitive ion transport systems in regulation of epithelial transport.  Comp. Biochem. Physiol. 148A:29-43 2007. PDF

58.    Marshall, W.S. Rapid regulation of ion in mitochondrion rich cells.  In. Fish Osmoregulation.  B. Baldisserotto, J.M. Mancera Romero and B.G. Kapoor, Ed. (Science Publishers, Enfield, 529pp) invited book chapter, pp395-426, (2007) 

59.    Marshall. W.S. and M. Grosell.  Ion transport and osmoregulation in fish. In: Physiology of Fishes, 3rd Edition, edited by D.H. Evans and J.B. Claiborne, CRC Press, Boca Raton FL. pp177-230. (601 pp total.) (2006) {NSERC and NSF}

60.    W.S. Marshall. Na+, Cl-, Ca2+ and Zn2+ transport by fish gills: retrospective review and prospective synthesis.  J. Experimental Zoology 293:264-283 (2002) Invited review PDF

61.    W.S. Marshall and T.D. Singer.  Cystic Fibrosis Transmembrane Conductance Regulator in teleost fish.  Biochim. Biophys Acta, Reviews in Biomembranes 1566: 16-27, 2002. {NSERC} PDF

62.    Marshall, W.S. Transport processes in isolated teleost epithelia: Opercular epithlium and urinary bladder.  Book Chapter in Fish Physiology Vol 14, T. Shuttleworth & C.M. Wood, eds., Academic Press NY pp 1-23 (1995) Invited book chapter

63.    Pequeux, A., R. Gilles and W.S. Marshall.  NaCl transport in gills and related structures: In: Advances in Comparative and Environmental Physiology I. NaCl Transport in Epithelia.  R. Greger, ed, Springer-Verlag, Berlin.  Pp 1-73 (1988) Invited book chapter.

 

INVITED CONFERENCE PRESENTATIONS

 

FULL PAPERS

 

64.    Marshall, W.S. and S.E. Bryson. Transport mechanisms of seawater chloride cells: An inclusive model of a multifunctional cell.  Comp. Biochem. Physiol. 119A:97-106 (1998).

65.    Marshall, W.S., S.E. Bryson and D.R. Idler.  Control of ion transport by the blood-testis barrier in brook trout.  1st Intl. Sympos. Fish Endocrinol. Fish Physiol Biochem. 7:331-336 (1989)

66.    Marshall, W.S.  Control of K+ secretion in the blood-testis barrier of brook trout.  In: Proceedings of the 3rd International Symposium on Reproductive Physiology of Fish.  D.R. Idler, L.W. Crim and J.M. Walsh, ed., Memorial Univ. Newfoundland, 264 (1987). (refereed conference paper)

67.    Marshall, W.S. and S.D. Klyce.  Control mechanisms of Cl‑ secretion in some vertebrate epithelia.  In Current Trends in Comparative Endocrinology  B. Lofts and W.N. Holmes, ed., Hong Kong Univ. Press, pp 971‑976 (1985).

68.    Marshall, W.S. and Bern, H.A.  Ion transport across the isolated skin of the teleost, Gillichthys mirabilis. In:  Epithelial transport in the lower vertebrates.  B. Lahlou, ed., Cambridge Univ. Press, pp. 337‑350 (1980).

 

PAPERS IN PREPARATION

 

Marshall, W.S., S.D. McCormick and T. Doyle.  Time course of adaptation of the euryhaline teleost Fundulus heteroclitus to fresh water.  In preparation

Marshall, W.S. and A. Epega.  CFTR expression in fish brain; ancient association with hypothalamic releasing factors. In preparation

 

OTHER CONTRIBUTIONS

INVITED CONFERENCE PRESENTATIONS, ABSTRACTS (not refereed)

69.    Marshall, W.S. Regulation of epithelial transport by osmotic cues.  (Invited Symposium paper, Soc. Exp. Biol. Conference, Glasgow, April 2009) in press

70.    **Katoh, F., H.P. *Main, B.N.G. *Lynch, R.R.F. Cozzi and W. S. Marshall. A novel functional regulation of NKCC1 in osmosensing chloride cells: involvement of FAK pY407 and mechanosensor integrin β1
Poster and selected poster talk. Gordon conference “Cellular Osmoregulation: sensors, transducers & regulators” Centre Paul Langevin, Aussois, France June 3-8, 2007,

71.    Marshall, W.S. Osmosensing mechanisms of ion transporting epithelial systems. (Invited Symposium paper, Soc Exp. Biol., Canterbury) April 2006.

72.    Marshall, W.S., E.K. Hoffmann, C.G. Ossum and R.R.F.Cozzi. Osmosensing chloride cells in Fundulus heteroclitus rapidly regulate ion transport by Na,K,2Cl cotransporter using p38 MAPK, JNK, PKC, SPAK, OSR1 and FAK.  (Invited symposium paper: Cell volume in health and disease, Copenhagen) Sept. 2005

73.    Marshall, W.S., C.G. Ossum, R.R.F. Cozzi and E.K. Hoffmann.  Osmosensing chloride cells in euryhaline fish gills:  How to adapt without really trying.  (Invited symposium paper, Soc. Exp. Biol. Conference, Barcelona) July 2005.

74.    Marshall, W.S. and E.K. Hoffmann. Rapid regulation of ion transport by mitochondria rich cells in opercular epithelium of an estuarine teleost. (Invited Symposium paper: Responses of fish to environmental changes, Taipei, Taiwan) August 2004.

75.    Marshall, W.S. CFTR Expression patterns in a euryhaline teleost fish.  (Symposium presentation in the Society for Integrative and Comparative Biology (SICB) 2003 meeting in Toronto.  I was also the symposium organizer: "Comparative Biology of CFTR")

76.    Marshall, W.S.  Control of anion transport by teleost chloride cells (Invited symposium presentation, Soc. Exp. Biol.  Southampton) 2003.

77.    Marshall, W.S., E. *Lynch, J.A. *Howard, R.R.F. Cozzi.  Immunocytochemical tracking of CFTR and NKCC transporters in chloride cells and intestine of Killifish: Changes with salinity adaptation. 5th Int Congress on the Biology of Fish (Vancouver, 2002) (5-page program abstract)

78.    Marshall, W.S. and T.D. Singer  Tracking and tracing ion transporters in teleosts using techniques by Hans Ussing and others.  (Invited symposium paper Soc. Exp. Biol. Swansea Wales) 2002.

 

OTHER MEETING ABSTRACTS (not refereed)

79.    *Strapps C.P., and Marshall, W.S.  Effects of Argulus sp. infestation on osmoregulatory abilities of the euryhaline teleost, Fundulus heteroclitus.  Cdn. Soc. Zool. Bull. 39:2:xx (in press)

80.    *Goudie, M.T. and Marshall W.S.  Cell volume regulation following hypotonic shock in mitochondria rich cells of the killifish (Fundulus heteroclitus) opercular epithelium.  Cdn Soc. Zool. Bull. 39:2:xx (in press)

81.    *Watters, K.D. and Marshall W.S. The role of focal adhesion kinase in regulation of ion transport in the teleost Fundulus heteroclitus.  Cdn. Soc. Zool. Bull. 39:2:xx (in press)

82.    *Watters, K.D., **Katoh, F., Cozzi, R.R.F., and Marshall, W.S.. CFTR Cl- channel is regulated by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensing mitochondria-rich ion transporting cells of killifish (Fundulus heteroclitus).  FASEB J. Abstract 934.16 in press (2008)

83.    *Watters, K.D. and Marshall W.S. The role of focal adhesion kinase in regulation of ion transport in the teleost Fundulus heteroclitus.  Poster presented at APICS Biology Undergraduate conference, March 2008.

84.    Marshall, W.S., H.P. *Main, F. **Katoh, R.R.F. Cozzi.  Differential phosphorylation of focal adhesion kinase (FAK) in hypotonic regulation of NKCC cotransporter in NaCl secretion by Fundulus heteroclitus.  FASEB J. 21:6:A1335 (abstract) 2007

85.    **Katoh, F., B.N.G. *Lynch, R.R.F. Cozzi and W.S. Marshall.  Phosphorylation of focal adhesion kinase (FAK) at tyrosine 407 and integrin β1 regulate NKCC cotransporter in Fundulus heteroclitus.  FASEB J. 21:6:A1337 (abstract) 2007

86.    Marshall, W.S., H.P. *Main, R.R.F. Cozzi and F. **Katoh.  Hypotonic regulation of NKCC in Killifish opercular membrane.  8th International Symposium on Fish Physiology. St. John’s NF. June 2006.

87.    *Strapps, C.P. Effects of Argulus funduli  infestation on osmoregulatory abilities of the euryhaline teleost Fundulus heteroclitus. Poster presented at APICS Biology undergraduate Conference April 2005 (program abstract)

88.    Marshall, W.S., C.G. Ossum, R.R.F. Cozzi and E.K. Hoffmann. Osmosensing chloride cells in Fundulus heteroclitus rapidly regulate ion transport via p38 MAPK, JNK, PKC, SPAK, OSR1 and FAK. Poster presented at IUPS/EB Conference San Diego, May 2004.  FASEB J. 19:6:A1234 (2004).

89.    Marshall, W.S., C.G. Ossum and E.K. Hoffmann. Osmosensing chloride cells rapidly regulate ion transport in an estuarine teleost fish. Bull. Cdn. Soc. Zool.  Program abstract (2004).

90.    Marshall, W.S. CFTR expression patterns in euryhaline teleost fish.  Symposium paper Soc. Int. Comp. Biol. Meeting, Toronto January 2003 (I was symposium organizer and presenter in a symposium called “Comparative Biology of CFTR”)

91.    Marshall, W.S. Control of chloride transport in teleostean chloride secreting cells.  Soc. Exp. Biol. Southampton U.K. (Invited symposium paper) (April 2003)

92.    Marshall, W.S. and T.D. Singer  Tracking and tracing ion transporters in teleosts using techniques by Hans Ussing and others.  Soc. Exp. Biol. Swansea Wales 2002 (Invited symposium paper).

93.    Marshall W.S. Trafficking of CFTR anion channels in chloride cells of Killifish, Fundulus heteroclitus during acclimation to sea water. 7th International Symposium on Fish Physiology Vancouver, BC July 2002. (Invited Symposium paper)

94.    Marshall, W.S., K. *Daborn, T. *Doyle, S.D. McCormick and T. Singer.  Regulation of ion transport by chloride cells of euryhaline teleost gill epithelium: Hormones, tonicity and cytoskeleton. European Society of Comparative Biochemistry and Physiology, Liege, Belgium 2001 (Invited symposium paper)

95.    *Doyle, T., S.D. McCormick, W.S. Marshall.  Freshwater adaptation in killifish Fundulus heteroclitus Rapid changes in morphology and function of chloride cells.  Program Abstract, CSZ 2000.

96.    *Daborn, K. and W.S. Marshall.  Determination of rate limiting transport steps by volume changes in teleost chloride cells.  Program abstract, Bull. CSZ 2000.

97.    Singer, T.D., Marshall, W.S., Schulte, P.M., Higgins, C.F. and McKinley, R.S.  Genomic organization of a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) homolog in a euryhaline teleost, Fundulus heteroclitus.  Comp. Biochem. Physiol.  124A:S60 1999 (abstract)

98.    *Daborn, K. and Marshall, W.S.  Dynamics of chloride cell – pavement cell interactions during abrupt salinity change.  Comp. Biochem. Physiol. 124A:S136 1999 (abstract).

99.    Marshall, W.S., S.E. Bryson and T. *Luby.  Control of epithelial Cl- secretion by basolateral osmolality in the euryhaline teleost Fundulus heteroclitus.  FASEB. J. abstract 1998

100.Marshall, W.S., S.E. Bryson, T. *Emberley and S.D. McCormick.  Time course of seawater adaptation in a strongly euryhaline teleost: A multivariate analysis.  Bull. C.S.Z. program abst. 1998

101.Marshall, W.S. and S.E. Bryson  Genistein inhibits Cl- secretion by teleostean chloride cells, a novel CFTR containing epithelial system.  FASEB Journal 11:4, Abst 1773, 1997

102.Marshall, W.S., S.E. Bryson and T. *Luby.  Osmotic swelling of chloride cells and autoregulation of Cl- secretion by Cl- cells.  Bull. C.S.Z. Program abstract 1997

103.Burgess, D.W., M.J. O'Donnell, W.S. Marshall and C.M. Wood.  Freshwater teleost ionoregulation: The search for an in vitro model.  Bull. C.S.Z. Program Abstract 1997

104.Marshall, W.S., S.E. Bryson, C.M. Liedtke and J. *Gillis.  Neural modulation of teleostean Cl- cell function: alpha-2-adrenoceptors mediate inhibition of Cl- secretion via Ca2+/IP3.  Bull. C.S.Z. Program abstract 1996

105.Marshall, W.S. and S.E. Bryson.  Chloride cell ion transport mechanisms.  Invited symposium paper, Soc. Exp. Biol., Lancaster UK 1996.

106.Marshall, W.S. Mechanisms and cost of ion regulation in a euryhaline teleost Soc. Exp. Biol. meeting 1995, St. Andrews' (program abstract).)

107.Marshall, W.S., S.E. Bryson, C.M. Liedtke and J. *Gillis.  Neural modulation of chloride cell function: α2-adrenoceptors modulate Cl- secretion via Ca2+ ans IP3. Soc. Exper. Biol. meeting abstract 1995.

108.Marshall, W.S. Low conductance anion channel activated by cAMP in teleostean chloride secreting cells. Soc. Exp. Biol. meeting Swansea, 1994 (program abstract)

109.Marshall, W.S. and S.E. Bryson.  Calcium and chloride secretion in seawater fish epithelia.  Soc. Exp. Biol. meeting, Canterbury, 1993 (program abstract)

110.Marshall, W.S. and S.E. Bryson.  Calcium: Intracellular mediator and transported ion in mitochondria rich cells of killifish opercular epithelium.  XII Int. Sympos. Comp. Endocrinol. 1993 (program abstract).

111.Marshall, W.S., J. *Burghardt and S.E. Bryson.  Ca2+ transport by mitochondria rich cells in the opercular epithelium of freshwater adapted killifish.  Bull. Cdn. Soc. Zool. 24:2:77, 1993 (abstract)

112.Bryson, S.E. and W.S. Marshall.  α2-adrenergic inhibition of chloride transport via a pertussis toxin insensitive pathway in teleostean chloride cells.  Bull. Cdn. Soc. Zool. 23:2:34, 1992

113.Marshall, W.S., S.E. Bryson, and D.R. Idler  Gonadotropin action on sperm duct epithelium: Ion transport stimulation mediatred by cAMP and Ca2+. 2nd Int. Sympos. Fish Endocrinol. St. Malo, France 1992.

114.Wood, C.M. and W.S. Marshall Ion transport by isolated skin of freshwater rainbow trout.  5th Int. Sympos. Fish Physiol. Ondense, Denmark, 1991 (Abstract)

115.Marshall, W.S., S.E. Bryson and D.R. Idler.  Calcium and cyclic AMP mediation of gonadotropin stimulation of ion transport.  Bull. Cdn. Soc. Zool. 22:2:44, 1991 (Abstract)

116.Marshall, W.S. and J.W. Hanrahan.  Anion channels in the apical membrane of cultured corneal epithelium: A patch clamp study.  Invest. Ophthalmol. Vis. Sci. 31:441 1990 (Abstract)

117.Marshall, W.S. and J.W. Hanrahan.  Outwardly-rectifying anion channel in the apical membrane of mammalian corneal epithelium in culture.  Amer. Zool. 29:127A 1989 (Abstract)

118.Marshall, W.S., S.E. Bryson and D.R. Idler.  Hormonal control of ion transport by brook trout sperm duct epithelium.  Soc. Exp. Biol. Meeting, Edinburgh 1989 (Abstract)

119.Marshall, W.S., R.J. *Fisher and S.E. Bryson.  Measurement of intracellular pH of epithelial in situ by microspectrofluorometry: Application to ion transport in teleosts.  Soc. exp. Biol. Meeting Edinburgh, UK, 1989 (program abstract)

120.Marshall, W.S. Intracellular pH of trout urinary bladder epithelial cells in situ by microspectrofluorometry:  Relations to Na+-H+(NH4+) and anion exchanges.  Proceedings 2nd Intl Congress Comp. Physiol. and Biochem. 1988. (Abstract)

121.Marshall, W.S. and S. Bryson.  Regulation of intracellular pH in urinary bladder epithelial cells from brook trout (Salvelinus fontinalis).  Bull. Can. Soc. Zool. 1988 (Abstract)

122.Marshall, W.S.  K+ secretion and Na+ absorption by the blood-testis of brook trout (Salvelinus fontinalis)  Bull. Can. Soc. Zool. 18:2:20, 1987 (Abstract).

123.Marshall, W.S.  Solute permeation through the paracellular shunt in brook trout urinary bladder epithelium.  Bull. Cdn. Soc. Zool., 17:2:30, 1986 (Abstract).

124.Marshall, W.S.  Transport and control in teleost gill, opercular and skin epithelia:  Recent Advances.  IUPS Banff satellite symposium "Membrane transport and control:  Comparative Mechanisms" 1986 (Abstract).

125.Marshall, W.S.  Urinary bladder of the brook trout:  Ion transport in a high resistance epithelium.  Federation proc. 43:444, 1984.  (Abstract)

126.Klyce, S.D., W.S. Marshall and C.E. Crosson.  Paracellular pathway in the rabbit corneal epithelium.  In: paracellular pathway and junctions in ocular tissues.  Pouliquen/raviola symposium 1984 (Abstract)

127.Marshall, W.S. and S.D. Klyce.  Serotonin stimulation of ion transport by the corneal epithelium:  A microelectrode study.  Invest. Ophthalmol. Vis. Sci. 22:100, 1982.  (Abstract)

128.Marshall, W.S. and S.D. Klyce.  Transport effects of serotonin in rabbit corneal epithelium.  Federation Proc. 41:1263, 1982.  (Abstract)

129.Marshall, W.S. and S.D. Klyce.  Membrane resistances in rabbit corneal epithelium.  Federation Proc. 40:3:370, 1981.  (Abstract)

130.Klyce, S.D., K.A. Palkama, W.S. Marshall, S. Huhtaniitty, and K.P. Mann:  evidence for serotonergic receptors in corneal epithelium.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 20:3:194, 1981.  (Abstract)

131.Marshall, W.S. and S.D. Klyce.  Epithelial membrane resistance in the rabbit cornea.  Invest. Ophthalmol. Vis. Sci. (Suppl.) 20:3:195, 1981.  (Abstract)

132.Marshall, W.S.:  Effects of high external salt on ion transport by marine fish skin. Federation Proc. 39:285, 1980.  (Abstract)

133.Marshall, W.S.:  Active transport of rubidium, a tracer of potassium, across the isolated skin of a marine teleost.  Amer. Zoologist 20:735, 1980 (abstract).

134.Klyce, S.D. and W.S. Marshall.  Membrane and paracellular pathway resistances in rabbit corneal epithelium.  In:  intracellular activities and potentials in epithelia of the eye.  Proc. Int. Soc. Eye research.  1:89, 1980 (abstract). 

135.Marshall, W.S. and Bern, H.A.  Active chloride transport by isolated fish skin:  Inhibition by the caudal neurohormone urotensin II.  Federation Proc. 38:3:1057, (abstract), 1979.

136.Marshall, W.S.:  Effects of hormones on ion transport across isolated skin preparations from a marine teleost.  In:  comparative endocrinology.  Proceedings of the 8th international symposium on comparative endocrinology, P.H. Gilliard and H.H. Boer, eds.  Elsevier/North Holland, Amsterdam, 1978, p. 238 (Abstract).

137.Marshall, W.S. and Nishioka, R.S.  Relation of chloride cells to anion transport by marine teleost skin.  Amer. Zoologist 19:944, 1979.  (Abstract) 

 

THESES

 

1.  BSc Hon: Marshall, W.S., 1973.  The effects of salinity on the lethal

temperatures of Fundulus heteroclitus L. and a study of some salt marsh pools.  Department of Biology, Acadia University, Wolfville, N.S., Canada.

 2.  Ph.D.: Marshall, W.S., 1977.  The functions and endocrine control of epithelial mucus secretion in the family Cottidae.  Department of Zoology, U.B.C., Vancouver, Canada.