|
Fig. 24
|
Centrifuge
tube containg sperm sample seperated on Percoll gradient. Note: Three bands
correspond to lancet (L) and carrier (C) parasperm and eusperm (E). Scale
bar = 10mm. |
|
Fig. 25
|
Sample
of lancet parasperm pipetted from top of gradient in fig. 24. Scale bar
= 50m m. |
|
Fig. 26
|
Sample
of carrier parasperm pipetted from middle of gradient in fig. 24. Scale
bar = 50m m |
|
Fig. 27
|
Sample
of eusperm pipetted from bottom of gradient in fig. 24. Scale bar = 50m
m |
|
Fig. 28
|
Sperm
plug taken from bursa copulatrix of female. Scale bar = 5m m. |
|
Fig. 29
|
Detail
of sperm plug in Fig. 28, showing mass of fused and unfused lancet parasperm
(L) and bound eusperm (arrowheads). Scale bar = 2m m. |
|
Fig. 30
|
Sperm
sample taken from seminal vesicles of males rapidly seperates in sea water
into components, eusperm (E), lancet (L) and carrier (C) parasperm. Scale
bar = 90m m. |
|
Fig. 31
|
Carrier
paraspermatid viewed with Nomarski optics. Scale bar = 10m m. |
|
Fig. 32
|
Same
carrier paraspermatid as in Fig. 31, stained with Hoechst's 33342 fluorescent
DNA stain, viewed under UV light. Note: Nuclear vesicles (arrows). Scale
bar = 10 m m. |