Figures
4
SEM of zygote with some hull cupules torn away showing channels that partition them (arrowheads). Note sperm (Sp) penetration at these and other locations where the hull is exposed. Closed cupules on an adjacent egg did not attract sperm. Bar = 40m m.
5
Part of figure 4 at higher magnification to show two fertilizing sperm that have penetrated the hull (white arrowheads) and have not entered through a micropyle. Note features of sperm such as basal mitochondria (M), main body of nucleus (N), and flagellum (F). A rounded structure attached to the flagellum may once have been the lateral mitochondrion (black arrow). Bar = 2m m.
6
Light micrograph of a 0.5m m section through a fertilizing sperm (Sp), with anterior filament (arrowhead) penetrating the hull (H) and vitteline layer (VL) at the base of a cupule. CG, Cortical granules; Y, yolk granules. Bar = 2m m