Figures
10
TEM of a section perpendicular to the oocyte membrane of a zygote illustrating pores (arrowheads) through the vitelline layer (VL). Each pore leads to the tip of a single egg microvillus (Mv). Note section of sperm nucleus (Sp) within one pore. H, hull. Bar = 0.5m m
11
TEM of fertilization. Anterior filament of sperm has fused with a single microvillus of the oocyte membrane (arrowhead), and nuclear chromatin (NC) is being injected into the egg. One cortical granule has been released by exocytosis (Ex), and two others (CG) are adjacent to the egg membrane. Note also the main body of sperm nucleus (N), hull (H), vitelline layer (VL), apparent fertilization cone (FC), and yolk granules (Y). Bar = 1 m m.
12
Site of fusion of sperm anterior filament with egg microvillus (Mv), showing sperm chromatin (arrowheads) entering egg cytoplasm devoid of nuclear membrane. FC, apparent fertilization cone. Bar = 0.1m m.