Biology 304
Metabolism Lab

Remember, there's a difference between oxygen consumption and metabolic rate. Mass-specific oxygen consumption is one index of metabolic rate (what is metabolic rate?). What are other indices of metabolic rate? Refer to the following figures in your text for pertinent results and the accompanying discussion for explanations: 5.8, 5.9 and 5.10.

Click for a full-sized version.
The graph illustrates the oxygen consumption for a number of endotherms under basal conditions. Where on this graph would you plot a 1 kg snake under basal conditions? Where would you plot this same snake if it was slithering away from a 4 kg fox that was chasing it? Where would you plot the fox? Please note that both axes are logarithmically-scaled.

Just so we're clear...the graph is the whole thing shown above; it is made up of axes (including numbers and labels), one curve, and grid lines (which I normally don't like).

Apparatus

 

Figure 1. The spirometre which can be used to measure CO2 evolution; the tracing on the right uses the modified protocol we used in lab starting in 2012; from that tracing, you should be able to measure Tidal Volume, Vital Capacity, Expiratory Reserve Volume, and Inspiratory Reserve Volume. The volume between the closest-spaced horizontal lines is 50 ml. Between the darkest horizontal lines is one litre. Stuck? See the digital tracings, below. What is the mathematical relationship between these four subdivisions of the lung?

 

  click for body surface area nomogram

Body Surface Area

click for heat production nomogram

Heat Production

 
Figure 2. A spirometre tracing for an hypothetical 15 year old boy that stands 105 cm tall and weighs 60 kg.  

Figure 3. A spirometre tracing of a 51.5 kg, 20 year old lady who stands 172 cm tall.

   

click for percent comparison nomogram

Percent Difference

 

The BTPS factor is 1.080, nomograms are available by clicking on their thumbnails.  
 
 

Figure 6. The digital versions of spirometry. Note that the x- and y-axis scales are different in the two images.

Blood-glucose data are available here:

 

Thanks to all who contributed their data!

 

R.F. Lauff
Department of Biology
St. Francis Xavier University

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