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Summer research assistant Karen Long prepares to extract peptides from natural materials which will then be tested for antibacterial activity. | ||||
| Research assistant Karen Long (left) and Biology honours student Catherine Hynes-Doherty (right) are seen here preparing electrophoretic materials. Catherine is comparing wild type and isogenic S layer deficient mutant strains of Campylobacter fetus to determine whether the S layer enhances C. fetus invasion of intestinal epithelial cells, as well as the effect of the S layer on intracellular survival and replication of C. fetus. |
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My research examines the molecular interactions between bacteria and mucosal host tissue. Campylobacter fetus, a pathogen of humans and animals is examined during interactions with model host cells. I use a morphologic approach using transmission and scanning electron microscopy and more recently fluorescence microscopy to visualize bacterial-host cell interactions. Bacterial strains are genetically manipulated to investigate specific virulence components. Here I am preparing host cells for use in adherence and invasion assays. | ||||
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